Localization of the major immunogen and other glycoproteins of the louse Polyplax spinulosa

Publication Type:Journal Article
Year of Publication:1994
Authors:P. Volf
Journal:International Journal for Parasitology
Pagination:1005 - 1010
Date Published:1994
Keywords:antibody response, antigen, antigens, glycoprotein, guinea pigs, immunization, Infestation, louse, Phthiraptera, Polyplax, protein, rat, serum

A 31-kDa immunogenic glycoprotein of Polyplax spinulosa was localized on louse sections using indirect immunofluorescence with specific antibodies of louse-infested rats and using fluorescein-labelled lectins. Antibodies of infested rats reacted specifically with midgut epithelium and semi-digested content of the gut. They did not bind to other louse tissues including salivary glands. The lectins concanavalin A and wheat germ agglutinin bound strongly to the immunogen, the gut and its semidigested contents. Electrophoretic and blotting analysis showed the presence of a 31-kDa glycoprotein component reacting with specific rat antibodies, concanavalin A and wheat germ agglutinin in louse faeces. The result indicates that the 31-kDa immunogen is secreted by the midgut epithelium into the gut lumen and faeces. We suggest that rats become immunized by scratching the lice faeces into the skin.

Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith