Production of Bartonella genus-specific monoclonal antibodies

Publication Type:Journal Article
Year of Publication:2001
Authors:Z. Liang, La Scola, B., Lepidi, H., Raoult, D.
Journal:Clinical and Diagnostic Laboratory Immunology
Pagination:847 - 849
Date Published:2001
ISBN Number:1071-412X
Keywords:animals, Antibodies, Bacterial/biosynthesis/immunology, Antibodies, Monoclonal/biosynthesis/immunology, Antigens, Bacterial/immunology, Bartonella henselae/immunology, Bartonella quintana/immunology, Bartonella/immunology, humans, Lice/microbiology, mice, Mice, Inbred BALB C, Rabbit

Monoclonal antibodies (MAbs) which react with heat-resistant proteins with molecular masses of 32 to 33 kDa of 14 different Bartonella species were produced. These antibodies did not react with antigens of 26 diverse bacterial strains by microimmunofluorescence assay except MAb B3D4, which reacted with Chlamydia psittaci and Chlamydia trachomatis at low titers. The identification of a common Bartonella antigenic protein will make it possible to later produce a diagnostic antigen by cloning and expressing it in Escherichia coli. Moreover, these MAbs allow all Bartonella species to be identified to the genus level.

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